UNIT I : Inroduction to recombinent DNA technology

Basic Principles
Importance of rDNA Technology
Historical developments – Early developments of Genetics leading to rDNA
The advent of gene cloning
What is gene cloning?
General outline of cloning technology
Vehicles of cloning
Technique for handling DNA
Different cloning Vectors
Why rDNA technology is so important?
The impact of rDNA on Industry – Biotechnology

UNIT II: Cloning vectors and enzymes used in rDNA
technology

Restriction enzymes
Modifying Enzymes
Different Cloning Vectors
Plasmids
Bactriophages, Cosmits

UNIT III : Strategies of cloning

Genomic DNA Libraries
cDNA Libraries
Selection and Screening of libraries

UNIT IV : Analysis of rDNA clones

Characterization of clones
Immunochemical
Nuclie acid hybridization
Invitro amplification
Mapping of restriction sites
Sequencing


UNIT V : Expression of cloned genes

Studying the transcript of a cloned gene using different techniques
Hybrid release translation(HRT)
Hybrid arrest release translation(HART)
Cell free translation Systems
Application of rDNA technologies