Genetics Practical Syllabus

1. Isolation of auxotrophic mutants in E . Coli.

2. Fluctuation tests.

3. Gene mapping by conjugation in E . Coli.

4. Gene mapping in Drosophila. ( three factor crosses with D. Melanogaster.)

5. Tetrad analysis in Neurospora.

6. Genes involved in lactose operon in E . Coli.



Microbiology


1. Isolation of bacteria - Serial dilutions, plating techniques.

2. Gram Staining , Spore Staining, Motility - test etc.

3. Fermentative reactions ( Sugar fermantation test).

4. Catalase, urease test.

5. Growth Curve.

6. Fermentative production of alcohol, an organic acid.

7. Phage titrations.